大环内酯类抗生素对大鼠肝移植后缺血再灌注损(7) -电力系统保护与控制杂志社投稿

一、本刊要求作者有严谨的学风和朴实的文风，提倡互相尊重和自由讨论。凡采用他人学说，必须加注说明。二、不要超过10000字为宜，精粹的短篇，尤为欢迎。三、请作者将稿件（用WORD格式）发送到下面给出的征文信箱中。四、凡来稿请作者自留底稿，恕不退稿。五、为规范排版，请作者在上传修改稿时严格按以下要求： 1．论文要求有题名、摘要、关键词、作者姓名、作者工作单位（名称，省市邮编）等内容一份。 2．基金项目和作者简介按下列格式：基金项目：项目名称（编号）作者简介：姓名（出生年－），性别，民族（汉族可省略），籍贯，职称，学位，研究方向。 3．文章一般有引言部分和正文部分，正文部分用阿拉伯数字分级编号法，一般用两级。插图下方应注明图序和图名。表格应采用三线表，表格上方应注明表序和表名。 4．参考文献列出的一般应限于作者直接阅读过的、最主要的、发表在正式出版物上的文献。其他相关注释可用脚注在当页标注。参考文献的著录应执行国家标准GB7714-87的规定，采用顺序编码制。

大环内酯类抗生素对大鼠肝移植后缺血再灌注损(7)

作者:

关键词:

摘要：

海南省自然科学基金资助项目(SQ2015ZRJJ0364)，项目负责人：常顺伍

BACKGROUND:Liver transplantation is the standard treatment for end-stage liver disease and liver ,ischemia-reperfusion injury can reduce the success rate of liver a limited number of liver donors are available for transplantation,how to reduce liver ischemia-reperfusion injury has become the primary issue in liver transplantation.

OBJECTIVE:To evaluate the effect of macrolide antibiotics on ischemia-reperfusion injury after liver transplantation in rats.

METHODS:Rat autologous orthotopic liver transplantation model was rats were randomly divided into macrolide antibiotics group and control group.In the macrolide antibiotics group,the donor liver was treated with macrolide antibiotics (60 mg/kg roxithromycin,20 mg/kg clarithromycin and 40 mg/kg erythromycin) 30 minutes before hepatectomy,and the above macrolide antibiotic mixture was injected into the portal vein immediately after orthotopic liver the control group,rats were pretreated with the same volume of saline for 30 minutes before hepatectomy,and the same volume of saline was injected into the portal vein immediately after orthotopic liver survival rate of the rats was observed within 7 days after liver serum aspartate aminotransferase and alanine aminotransferase activities were detected by automatic biochemical analyzer at 48 and 72 hours after liver staining and immunohistochemistry assay were used to detect the morphological changes of liver tissues and the number of Ki-67 positive cells in liver transplantation and western blot assay were used to detect the number of apoptotic hepatocytes and the expression of caspase-3 and cleaved caspase-3 proteins in liver transplantation rats, Kupffer cell number changes and levels of interleukin-6,interleukin-1β,and tumor necrosis factor-α in rat liver tissues after liver transplantation were detected by immunofluorescence and ELISA,respectively.

RESULTS AND CONCLUSION:Macrolide antibiotics increased the overall survival rate of liver transplanted rats,improved the dysfunction of transplanted liver,reduced the severity of ischemia-reperfusion injury after liver transplantation,increased the regenerative capacity of transplanted liver,reduced the number of apoptotic cells and the ratio of cleaved caspase-3/caspase-3 in the transplanted liver tissue,and decreased the number of Kupffer cells and the levels of interleukin-6,interleukin-1β and tumor necrosis factor-α in the transplanted the results indicate that macrolide antibiotics protect against ischemia-reperfusion injury in rats undergoing liver transplantation.

文章来源：《电力系统保护与控制》网址: http://www.dlxtbhykzzz.cn/qikandaodu/2021/0501/858.html